Detección del virus SARS-CoV-2 a través de la prueba de amplificación isotérmica mediada por bucle con transcripción reversa (RT-LAMP)
DOI:
https://doi.org/10.54104/saywa.v5n6.1506Palabras clave:
COVID-19, RT-LAMP, SARS-CoV-2Resumen
Resumen
El propósito del presente estudio fue estandarizar una metodología rápida y de simple ejecución para la detección de SARS-CoV-2, usando la técnica de amplificación de ácidos nucleicos mediada por bucle con transcripción inversa en condiciones isotérmicas (RT-LAMP, del inglés Reverse Transcription Loop-mediated isothermal amplification), para el diagnóstico de la COVID-19. La estandarización se realizó en muestras de ARN previamente confirmadas por RT-qPCR usando el protocolo Charité, Berlín, Alemania. Detallamos los pasos de la técnica y destacamos el uso del cebador S-123. En conclusión, la prueba RT-LAMP con el set de cebadores S-123 para SARS-CoV-2 en muestras de ARN, mostró un alto desempeño con relación a la técnica gold standard RT-qPCR.
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Derechos de autor 2024 Elizabeth Ruiz Marvez, Ana Luisa Muñoz Ramírez, Orlando Alfredo Torres García, Francisco Javier Vargas Ortíz, Juana Patricia Sánchez Villamil
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
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