Efecto de extractos de propóleos colombianos sobre la expresión génica de células cancerígenas
Keywords:
Acción citotóxica, Células tumorales, Extractos etanólicos, PropóleoAbstract
Propolis is a natural product collected and processed by bees that consists of a resinous mixture of chemical composition changing from region to region in the world. The antitumor effect of propolis from different geographical areas has been widely reported. In this work, we made a chemical characterization of four ethanol extracts of Colombian Propolis (EEP) and their cytotoxic action against canine osteosarcoma cells (OS) and fibroblasts derived from a primary canine culture was evaluated. In addition,changes in the gene expression of apoptotic, associated with inflammation and the cell cycle, were evaluated in these two cell types. The results indicate an antiproliferative activity of propolis on OSCA-8 tumor cell line, highlighting a greater cytotoxic effect in these cells with respect to fibroblasts. Furthermore, an increased in the expression of proinflammatory and proapoptotic genes and proapoptotic processes were found in cells treated with EPP.
Downloads
References
Castro, V., Negri, G., Salatino, A., y Bandeir, M. (2011). A new type of Brazilian propolis: prenylated benzophenones in propolis from Amazon and effects against cariogenic bacteria. Food Chemistry, 125, 966-972 http://doi.org/10.1016/j.foodchem.2010.09.089
Cinegaglia, N.C., Bersano, P.R., Bufalo, Sforcin1, J., 2012. Cytotoxic Action of Brazilian Propolis In Vitro on Canine Osteosarcoma Cells. Phytotherapy Research, 27, 1277-1281.
Freires, I. A., de Alencar, S. M., y Rosalen, P. L. (2016). A pharmacological perspective on the use of Brazilian Red Propolis and its isolated compounds against human diseases. European Journal of Medicinal Chemistry, 110, 267-279. http://doiI:10.1016/j.ejmech.2016.01.033.
Marcelo, F., David, C., Marcos, G., Fernanda, V., André, L., Bruno, B., Severino, M., Thiago, M., Pedro, L. (2016). Neovestitol, an isoflavonoid isolated from Brazilian red propolis, reduces acute and chronic inflammation: involvement of nitric oxide and IL-6. Nature Scientific Reports, 6,36401. http://DOI: 10.1038/srep36401.
Pardo Mora D, Santiago K, Conti B, de Oliveira Cardoso E, Conte F, Oliveira L, de Assis Golim M, Uribe J, Gutiérrez RM, Rey M, Popova M, Trusheva B, Bankova V, García O, Sforcin JM.(2019). The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples.Phytother Res. 33(3):591-601.
Simpson, S., Dunning, M. D., de Brot, S., Grau-Roma, L., Mongan, N. P., y Rutland, C. S. (2017). Comparative review of human and canine osteosarcoma: morphology, epidemiology, prognosis, treatment and genetics. Acta Veterinaria Scandinavica, 59, 71. http://doi.org/10.1186/s13028-017-0341-9.
Sureerat, B., Songchan, P., Tanapat, P., Kriengsak, L., Preecha, P., Jisnuson, S., Chanpen, C. (2015). Cardanol isolated from Thai Apis mellifera proplis induces cell cycle arrest and apoptosis of BT-474 breast cancer cells via p21 upregulation. DARU JournalofPharmaceuticalSciencies, 23-55. http://DOI 10.1186/s40199-015-0138-1.
Zaccaria, V., Curti, V., Di Lorenzo, A., Baldi, A., Maccario, C., Sommatis, S., Daglia, M. (2017). Effect of Green and Brown Propolis Extracts on the Expression Levels of microRNAs, mRNAs and Proteins, Related to Oxidative Stress and Inflammation. Nutrients, 9(10), 1090. http://doi.org/10.3390/nu9101090.
Downloads
Published
-
Abstract245
-
PDF (Español)139
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Portal de Ciencia Abierta