Detection of SARS-CoV-2 virus by reverse transcription Loop-mediated isothermal amplification (RT-LAMP) assay

Autores

DOI:

https://doi.org/10.54104/saywa.v5n6.1506

Palavras-chave:

COVID-19, RT-LAMP, SARS-CoV-2.

Resumo

Abstract

The purpose of the present study was to standardize a fast and effortless methodology for the detection of SARS-CoV-2, by reverse transcription loop-mediated isothermal nucleic acid amplification technique (RT-LAMP) for COVID-19 diagnosis. Standardization was made in RNA samples previously confirmed by RT-qPCR using the Charité/Berlin protocol. We detail the steps of the technique and highlight use of S-123 primers. In conclusion, RT-LAMP with S-123 primers for SARS-CoV-2 in RNA samples, shows a high level of performance compared with gold standard technique RT-qPCR.

 

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Referências

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2024-02-04
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Como Citar

Ruiz Marvez, E., Muñoz Ramírez, A. L., Torres García, O. A., Vargas Ortíz, F. J., & Sánchez Villamil, J. P. (2024). Detection of SARS-CoV-2 virus by reverse transcription Loop-mediated isothermal amplification (RT-LAMP) assay. REVISTA SAYWA, 5(6), 17–26. https://doi.org/10.54104/saywa.v5n6.1506

Edição

v. 5 n. 6 (2023)

Seção

Ciencia Aplicada

Licença

Copyright (c) 2024 Elizabeth Ruiz Marvez, Ana Luisa Muñoz Ramírez, Orlando Alfredo Torres García, Francisco Javier Vargas Ortíz, Juana Patricia Sánchez Villamil

Creative Commons License

Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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